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The second response cycle in NO synthesis includes O2 binding to ferrous NOS to form the Fe, IIO2 intermediate, followed by oxidation of NOHA to generate ferric enzyme, NO, citrulline, and water as products (Fig. 9) - UV/Vis/NIR. As attracted Fig - https://pblc.me/pub/3fc0b3e264b77b. 9, the reaction important link might likewise include electron transfer from H4B to the Fe, IIO2 intermediate

l9 As shown in Fig. 9, this might be due to the fact that the H4B radical serve as an electron sink in a later point in the response, so regarding allow NOS to, Fig. 9. A model for NORA oxidation by NOSoxy in the single turnover response. Oxygen binds to the ferrous protein to form the Fe, IIO2 types, which upon receiving an electron from H4B forms a putative hemeperoxo intermediate that reacts with NOHA.